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除了我們自己的實力,包括經(jīng)驗豐富的專業(yè)科學(xué)家,技術(shù)人員和先進(jìn)的基礎(chǔ)設(shè)施,我們還與印度的其他三個主要研發(fā)團(tuán)隊合作。我們在這個行業(yè)工作了12年,我們的一些科學(xué)家擁有超過25年的經(jīng)驗。
根據(jù)客戶要求,我們提供負(fù)責(zé)項目的科學(xué)家和技術(shù)人員的簡歷,客戶可以直接與項目協(xié)調(diào)員溝通。一切都是嚴(yán)格保密的。
· 克隆,表達(dá)和大規(guī)模純化
· 敲除構(gòu)建體,酵母遺傳學(xué)
· 基于體外Ag-Ab的測定
· 基于細(xì)胞的分析
· 代謝紊亂
· 酶
· 炎癥
· 癌癥
· 中樞神經(jīng)系統(tǒng)(CNS)
· 心血管
PAP(焦磷酸解 -活化聚合)/ KlenTaq -S
PAP是一種*的DNA擴(kuò)增方法(Liu和Sommer:BioTechniques,29 [2000],1072-1080; Nucleic Acids Res,30 [2002],598-604),其中焦磷酸解 和聚合通過DNA聚合酶使用3連續(xù)偶聯(lián)。 '封閉的底漆。 焦磷酸解是DNA聚合的逆反應(yīng),其中在無機(jī)焦磷酸鹽存在下酶除去引物模板雙鏈體3'末端的封閉核苷酸(通常是雙脫氧)。在通過焦磷酸解作用除去封閉的核苷酸后,然后可以通過DNA聚合延長活化的引物。
由于PAP 的高特異性(1 / 3x10 11) ,該過程正成為 在大量過量野生型等位基因存在下檢測稀有突變,大雜合缺失,基因重復(fù)等的有力工具(Liu et al:Biotechniques,40 [2006],661-668)。理論上,PAP可以檢測3x10 11拷貝的野生型等位基因中的單個堿基突變。
PAP可以被用來作為一種替代或證實的實時PCR (Boon等人: Prenat DIAGN 27 [2007],932-937)
KlenTaq -S和PAP反應(yīng)。
已經(jīng)發(fā)現(xiàn)KlenTaq -S是 PAP反應(yīng)中許多研究小組的合適的熱穩(wěn)定 Taq DNA聚合酶。
KlenTaq -S由該基因的5'缺失和F667Y(Tabor和Richardson)突變表達(dá),編碼Thermus aquaticus DNA聚合酶。反復(fù)暴露于98 0在PR1反應(yīng)C緩沖區(qū)的酶不顯示活性的任何顯著損失。
KlenTaq - S的濃度: 10U /μl
建議的PAP反應(yīng)條件(25μl ):
50 mM Tris-HCl,7.8,16 mM硫酸銨,3.5 mM 氯化鎂, 25μM,每種四種dNTP,每種引物0.1μM,5-200 ng 模板,90μM無機(jī)焦磷酸鹽(Na 4 PPi),2%二甲基亞砜(DMSO) ),2.5 U KlenTaq -S。
騎行條件:
95 0 Ç 為 1分鐘(變性),然后
94 0 C持續(xù)15秒
60 0 C持續(xù)30秒
64 0 C持續(xù)30秒
68 ° C,1分鐘
72 0 C持續(xù)1-2分鐘,共25-45個循環(huán)
提供緩沖液 ( 10X PR1):
500 毫米 的Tris-HCl,pH值7.8,160 mM的 ( NH 4)2 SO 4,35 毫的MgCl 2,1.5毫克/毫升BSA
訂購信息:
訂貨號 :KTS-01
數(shù)量: 100μl或1000 U /小瓶
價格: $ 200 /小瓶(1000 U)
運費: 美國35美元(FedEx隔夜)
Jembio 產(chǎn)品目錄:
貨號 | 品名 | 規(guī)格 | 美金價 | 品牌 |
LA001 | Acid Phosphatase, Polyclonal anti-Human Affinity Purified | 5 mg | 1300 | jembio |
LA002 | Albumin, Polyclonal anti-Human Affinity Purified (ALB) | 10 mg | 800 | jembio |
LA003 | Albumin, Polyclonal anti-Human Affinity Purified (ALB) | 10 mg | 600 | jembio |
LA004 | Alpha Amylase, Polyclonal anti-Human (whole antiserum) | 50 ml | 900 | jembio |
LA005 | Alpha Fetoprotein, Polyclonal anti-Human (AFP) Whole Antiserum | 50 ml | 900 | jembio |
LA006 | Alpha-1 Antitrypsin, Polyclonal anti-Human Affinity Purified (A1AT) | 10 mg | 700 | jembio |
LA007 | Alpha-1 Antitrypsin, Polyclonal anti-Human Affinity Purified (A1AT) | 10 mg | 700 | jembio |
LA008 | Alpha-1-Acid Glycoprotein, anti-Human Affinity Purified (A1AG) | 10 mg | 750 | jembio |
LA009 | alpha-2-Macroglobulin, Polyclonal anti-Human affinity Purified (A2M) | 10 mg | 700 | jembio |
LA010 | anti-Feline Leukemia Virus p27, Monoclonal | 5 mg | 3000 | jembio |
LA011 | anti-Feline Leukemia Virus p27, Monoclonal-CMI | 5mg | 3000 | jembio |
LA012 | beta 2-Microglobulin ,anti-Human HRP Conjugated | 10 mg | 1400 | jembio |
LA013 | beta 2-Microglobulin, Polyclonal anti-Human Affinity Purified (B2M) | 10 mg | 650 | jembio |
LA014 | BNP Polyclonal anti-human affinity purified | 5 mg | 3000 | jembio |
LA015 | C-Reactive Protein (CRP), Polyclonal anti-Rat affinity purified | 1 mg | 1500 | jembio |
LA016 | C-Reactive Protein, Polyclonal anti-Human affinity purified (CRP) | 10 mg | 750 | jembio |
LA017 | C3, Polyclonal anti-Human Affinity Purified | 10 mg | 750 | jembio |
LA018 | C4 , Polyclonal anti-Human Affinity Purified | 10 mg | 750 | jembio |
LA019 | Cathepsin, Polyclonal anti-Human Affinity Purified | 5 mg | 1450 | jembio |
LA020 | Ceruloplasmin, Polyclonal anti-Human Affinity Purified | 10 mg | 750 | jembio |
LA021 | Elastase, anti-Human IgG Fraction | 100 mg | 500 | jembio |
LA021 | Elastase, anti-Human IgG Fraction | 100 mg | 500 | jembio |
LA022 | Elastase, Polyclonal anti-human (whole anti-serum) | 30 ml | 400 | jembio |
LA023 | Ferritin, Polyclonal anti-Human Affinity Purified | 10mg | 1750 | jembio |
LA024 | Goat anti-Human Whole Serum | 100 ml | 1750 | jembio |
LA025 | Haptoglobin Polyclonal anti-human (whole antiserum) | 10 ml | 500 | jembio |
LA026 | Haptoglobin, Polyclonal anti-Human Affinity Purified (Hp) | 10 mg | 1200 | jembio |
LA027 | Haptoglobin, Polyclonal anti-Human Affinity Purified (Hp) | 10 mg | 1200 | jembio |
LA028 | Heartworm,.Polyclonal anti-Canine Affinity Purified | 10 mg | 3000 | jembio |
LA029 | Heartworm,Polyclonal anti-Canine,HRP Conjugated | 10 mg | 3500 | jembio |
LA030 | Hemoglobin, Polyclonal anti-Human Affinity Purified | 30 ml | 750 | jembio |
LA031 | Hemoglobin, Polyclonal anti-Human Affinity Purified | 10 mg | 750 | jembio |
LA032 | IgA, Polyclonal anti-Human Affinity Purified | 10 mg | 750 | jembio |
LA033 | Kappa, Polyclonal anti-Human Affinity Purified | 10 mg | 1000 | jembio |
LA034 | Kappa, Polyclonal anti-Human Affinity Purified | 10 mg | 750 | jembio |
LA035 | Lactate Dehydrogenase , Polyclonal anti-Human Affinity Purified (LDH) | 10 mg | 3500 | jembio |
LA036 | Lactoferrin Polyclonal anti-Human Affinity Purified | 10 mg | 1300 | jembio |
LA037 | Lactoferrin, Polyclonal Whole anti-serum | 50 ml | 1000 | jembio |
LA038 | Lambda, Polyclonal anti-Human Affinity Purified | 10 mg | 1000 | jembio |
LA039 | Lambda, Polyclonal anti-Human Affinity Purified | 10 mg | 750 | jembio |
LA040 | Myeloperoxidase, Polyclonal anti-human affinity purified | 10 mg | 3500 | jembio |
LA041 | Myeloperoxidase, Polyclonal anti-Human affinity purified (MPO) | 10 mg | 3500 | jembio |
LA042 | Myoglobin Polyclonal anti-human affinity purified | 10 mg | 2500 | jembio |
LA043 | Myoglobin, Polyclonal anti-Human Affinity Purified | 10 mg | 5000 | jembio |
LA044 | Prostate Specific Antigen,(PSA) Polyclonal anti-Human Affinity Purified | 5 mg | 2300 | jembio |
LA045 | Rabbit anti-Human Whole Serum | 50 ml | 2000 | jembio |
LA046 | Retinol Binding Protein ,Polyclonal anti-Human Affinity Purified (RBP) | 10 mg | 3200 | jembio |
LA047 | Transferrin, Polyclonal anti-Human Affinity Purified | 10 mg | 750 | jembio |
LA048 | Troponin I, anti-human whole antiserum | 500 ml | 15000 | jembio |
1001 | KlenTaq1TM | 2,500 | 200 | jembio |
1001 | KlenTaq1TM | 25,000 | 1,800.00 | jembio |
1002 | DNA Polymerase, alpha | 50 | 200 | jembio |
| (Calf thymus) | 200 | 725 | jembio |
1004 | DNA Polymerase, alpha | 50 | 250 | jembio |
| (Human) | 200 | 900 | jembio |
1006 | DNA Polymerase, gamma | 50 | 260 | jembio |
| (Human) | 200 | 900 | jembio |
1008 | DNA Polymerase 1 | 500 | 70 | jembio |
|
| 2,500 | 280 | jembio |
1010 | Klenow Fragment | 1,000 | 280 | jembio |
| (DNA Polymerase) | 5,000 | 875 | jembio |
1011 | Klenow Fragment | 1,000 | 280 | jembio |
| (exo-) | 5,000 | 880 | jembio |
1012 | T4 DNA Polymerase | 1,000 | 400 | jembio |
|
| 2,500 | 835 | jembio |
1022 | Terminal Deoxynucleotidyl | 500 | 100 | jembio |
| Transferase | 2,500 | 450 | jembio |
1102 | AMV Reverse Transcriptase | 500 | 130 | jembio |
|
| 2,000 | 450 | jembio |
1104 | HIV Reverse Transcriptase | 500 | 420 | jembio |
|
| 2,500 | 1,340.00 | jembio |
1106 | Tth 111 Reverse Transcriptase | 100 | 75 | jembio |
|
| 500 | 300 | jembio |
1202 | DNA Ligase, E.coli | 500 | 120 | jembio |
|
| 2,500 | 500 | jembio |
1204 | T4 DNA Ligase | 200 | 75 | jembio |
|
| 2,000 | 300 | jembio |
1206 | Taq DNA Ligase | 1,000 | 110 | jembio |
|
| 5,000 | 450 | jembio |
1302 | Alkaline Phosphatase | 1,000 | 65 | jembio |
|
| 5,000 | 275 | jembio |
1304 | Exonuclease III | 5,000 | 30 | jembio |
|
| 2,500 | 90 | jembio |
1306 | Mung Bean Nuclease | 2,000 | 50 | jembio |
|
| 10,000 | 200 | jembio |
1308 | RNase I | 10,000 | 150 | jembio |
|
| 25,000 | 300 | jembio |
1312 | S1 Nuclease | 10,000 | 65 | jembio |
|
| 50,000 | 275 | jembio |
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6:我們還是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等幾十家國外公司授權(quán)代理。
7:我們還是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知*批發(fā),歡迎合作。
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