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安全 · 高敏 · 便捷——GelRed®核酸染料(貨號(hào)41003)多維度解析!?

更新時(shí)間:2025-06-27      點(diǎn)擊次數(shù):47

GelRed-1-400x400.png.webp

?? 產(chǎn)品詳細(xì)介紹

GelRed® Nucleic Acid Gel Stain(貨號(hào)41003) 是Biotium公司推出的革命性核酸染色試劑,專(zhuān)為瓊脂糖和聚丙烯酰胺凝膠中的DNA/RNA檢測(cè)設(shè)計(jì)。  

- 規(guī)格:10000× 水溶液(0.5mL/1mL),兼容各類(lèi)電泳設(shè)備。  

- 工作原理:通過(guò)嵌入雙鏈核酸后發(fā)射熒光(激發(fā)最大值:~300/495nm,發(fā)射最大值:~600nm),與EB(溴化乙錠)相似但更安全。  

- 安全性認(rèn)證:經(jīng)Ames測(cè)試驗(yàn)證無(wú)誘變性,可通過(guò)廢棄物常規(guī)處理流程(如活性炭/漂白劑降解)。  

- 兼容性:適用于紫外(UV)或藍(lán)光透射儀(470-520nm),且與TAE、TBE等緩沖液兼容。  

---

? 核心優(yōu)勢(shì)  

1. ?? 安全性高

   - 零致癌風(fēng)險(xiǎn):不穿透細(xì)胞膜,無(wú)法進(jìn)入生物體,安全性遠(yuǎn)高于EB(IARC 1類(lèi)致癌物)!  

   - 廢棄物直排:實(shí)驗(yàn)廢液無(wú)需特殊處理(注:需符合當(dāng)?shù)丨h(huán)保法規(guī))。  

2. ?? 超高靈敏度  

   - 檢測(cè)限低至0.1ng DNA/條帶,靈敏度與EB相當(dāng),遠(yuǎn)超SYBR® Green I!  

   - 熒光信號(hào)穩(wěn)定>12小時(shí),無(wú)需脫色,結(jié)果清晰無(wú)背景干擾。  

3. ?? 操作便捷  

   - 即用型溶液:直接加入熔融瓊脂糖(55-60℃)中混勻,或電泳后浸泡染色(推薦濃度:1×)。  

   - 節(jié)省時(shí)間:染色3-5分鐘即可成像,比EB快50%!  


? 購(gòu)買(mǎi)高頻疑問(wèn)Q&A  

Q1:GelRed®溶劑是水還是DMSO?  

 答:貨號(hào)41003為水溶液,無(wú)需稀釋?zhuān)苯邮褂酶踩?nbsp; 

Q2:能用于<2%的高濃度膠嗎?  

答:兼容!1-4%瓊脂糖凝膠均可使用,染色均勻無(wú)沉淀。  

Q3:工作濃度是多少?  

答:推薦1×濃度:每100mL膠加10μL原液(10000×),成本僅需幾毛錢(qián)/次!  

Q4:藍(lán)光 vs 紫外激發(fā),哪個(gè)更好?  

答:藍(lán)光激發(fā)更優(yōu)!減少DNA損傷,且信號(hào)強(qiáng)度提升20%(配橙/紅色濾光片)。  

Q5:可否用于預(yù)制膠(pre-cast gel)?  

答: 避光4℃儲(chǔ)存,穩(wěn)定性>2年;避免反復(fù)凍融!


(注:本文內(nèi)容基于品牌公開(kāi)資料及行業(yè)常規(guī)信息整理,具體以品牌信息文檔為準(zhǔn)。)


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貨號(hào)

品名

規(guī)格

品牌

10030

FDP (high purity)

5 mg

Biotium

20015

CF®488ADonkeyAnti-RabbitIgG(H+L),HighlyCross-Adsorbed,2mg/mL

500uL

Biotium

20092

Donkey Anti-Rat IgG (H+L), Highly Cross-Adsorbed

0.5mL

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20282

CF®647 Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL

500uL

Biotium

20302

Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed (Min X Rat)

0.5mL

Biotium

20432

CF®488A Rabbit Anti-FLAG

100 uL

Biotium

22003

Mini Cell Scrapers

200ST

Biotium

22010

10XFishGelatinBlockingAgent

100ML

Biotium

23001

EverBrite™ Mounting Medium

1ml

Biotium

23005

CoverGrip™CoverslipSealant

15mL

Biotium

23007

TrueBlack® Lipofuscin Autofluorescence Quencher, 20X in DMF

1mL

Biotium

23012

TrueBlack® IF Background Suppressor System (Permeabilizing)

200 assays

Biotium

29022

WheatGermAgglutinin,CF®488AConjugate

5x1mg

Biotium

29050

Cheetah™ Hot Start Taq DNA Polymerase

500EU

Biotium

29051

EvaEZ™ Fluorometric Polymerase Activity Assay Kit

2 x 1 mL

Biotium

29052

ROX Passive Reference Dye

5ml

Biotium

30023

CellBrite™RedCytoplasmicMembraneDye

1ML

Biotium

30024

CellBrite™ Blue Cytoplasmic Membrane-Labeling Kit

1KIT

Biotium

30029

NucView® 488 Caspase 3 Assay Kit

1KIT

Biotium

30072

NucView® 488 and RedDot™ 2 Apoptosis & Necrosis Kit (100 assays)

1KIT

Biotium

30089

CellBrite™ Fix 640 Membrane Labeling Kit

1KIT

Biotium

30090

CellBrite™ Fix 488 Membrane Labeling Kit

1KIT

Biotium

31000

EvaGreen®, 20X in Water, 5x1 mL

5x1ml

Biotium

31019

EvaGreen®, 2000X in DMSO

50uL

Biotium

31038

PMA Enhancer for Gram Negative Bacteria, 5X Solution

16 mL

Biotium

31075

Viability PCR Starter Kit with PMA

1kit

Biotium

31077

EvaGreen® Plus, 20X in Water, 5x1 mL

5ml

Biotium

40002

dUTP CF® Dye Conjugates

25 nmol

Biotium

40008

CF®488A-dUTP,LyophilizedPowder

25nmol

Biotium

40012

DMAO, 2mM in DMSO

1ml

Biotium

40013

PMA (Propidium Monoazide)

1mg

Biotium

40019

PMA (Propidium Monoazide)

100uL

Biotium

40021

5-Aminoallyl-UTP, Sodium Salt, 10 mM in TE Buffer

100ul

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40027

dCTP CF® Dye Conjugates

25 nmol

Biotium

40035

Biotin-11-CTP, 10 mM Solution

25uL

Biotium

40050

Ethidium homodimer III

1mg

Biotium

40061

RedDot™2 Far-Red Nuclear Stain, 200X in DMSO

250ul

Biotium

40069

PMAxx™ Dye, 20 mM in H2O

100ul

Biotium

41001

GelRed® Nucleic Acid Gel Stain, 3X in Water

4L

Biotium

41002

GelRed® Nucleic Acid Gel Stain 10.000X in DMSO

0.5ML

Biotium

41003

GelRed® Nucleic Acid Gel Stain

0.5ml

Biotium

41004

GelGreen® 10,000X in DMSO

0.5 mL

Biotium

41005

GelGreen® Nucleic Acid Gel Stain, 10,000X in Water

0.5ML

Biotium

80014

Hydroxystilbamidine (Fluoro-Gold™)

10mg

Biotium

80110

CF®488A-Dextran 10,000 MW, Anionic and Fixable

1MG

Biotium

80112

CF®555-Dextran10,000MW,AnionicandFixable

1MG

Biotium

90066

Biotin-XX MTSEA

10mg

Biotium

91020

MTSES (2-Sulfonatoethyl methanethiosulfonate sodium salt) is a negatively charged MTS reagent.

100mg

Biotium

92120

CF®488A,succinimidylester

1UMOL

Biotium

#70037

SCAS

100 mg

Biotium

00005-100ug

α-Bungarotoxin, CF® Dye and Other Conjugates

100ug

Biotium

00010-1

Alpha-Bungarotoxin

1MG

Biotium

00034-T

Phalloidin,CF®405M

50U

Biotium

00042-T

Phalloidin, CF®488A

50U

Biotium

00044-T

phalloidin, CF568

50U

Biotium

10110-50uG

Coelenterazine (ALSO KNOWN AS NATIVE COE

50uG

Biotium

10111-50uG

Coelenterazine H

50uG

Biotium

10125-50uG

Coelenterazine 400A (DEEPBLUEC, A TRADEM

50uG

Biotium

20022-1mg

CF®488A Goat Anti-Human IgG (H+L), Highly Cross-Adsorbed

1mg

Biotium

20071-1

CF®488A Rabbit Anti-Human IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL

50ul

Biotium

20074-1mg

CF488A 驢 抗 人 IgG (H+L), highly cross-adsorbed

1mg

Biotium

20104-1

CF®568 Goat Anti-Chicken IgY (IgG) (H+L), Highly Cross-Adsorbed, 2 mg/mL

50ul

Biotium

20813-50uL

Goat Anti-Rabbit IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM

50uL

Biotium

21003-1L

One-Step Blue Protein Gel Stain, 1X

1L

Biotium

21004-1L

One-Step Lumitein™ Protein Gel Stain, 1X

1L

Biotium

21005-1L

One-Step Blue® Protein Gel Stain

1L

Biotium

23005-1

CoverGrip™ Coverslip Sealant Refill

100ml

Biotium

23014-T

TrueBlack® Plus Lipofuscin Autofluorescence Quencher, 40X in DMSO

50ul

Biotium

29022-1

WheatGermAgglutinin,CF®488AConjugate

1mg

Biotium

29023-1

WheatGermAgglutinin,CF®594Conjugate

1mg

Biotium

29026-1

CF®640R WGA

1mg

Biotium

29027-1

CF®405S WGA CF®405S 404/431 nm

1mg

Biotium

29028-1

WheatGermAgglutinin,CF®405MConjugate

1mg

Biotium

29091-10mg

R-Phycoerythrin (RPE), lyophilized solid, ammonium sulfate free

10mg

Biotium

29092-10mg

Allophycocyanin (APC), cross-linked lyophilized solid, ammonium sulfate free

10mg

Biotium

30020-T

ATP-Glo™ Bioluminometric Cell Viability Assay Kit (50 assays)

1KIT

Biotium

30028-L3

Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) 10 x 1000 Assays

10000TST

Biotium

30029-T

NUCVIEW 488 CASPASE 3 ASSAY KIT, TRIAL SIZE 1ST

1ST

Biotium

30089-T

CellBrite™ Fix 640 Membrane Labeling Kit, Trial Size

1KIT

Biotium

30090-T

CellBrite™ Fix 488 Membrane Labeling Kit, Trial Size

1KIT

Biotium

30096-T

MemBrite™ Fix 594/615 Cell Surface Staining Kit, 100 Reactions

1KIT

Biotium

30114-T

ExoBrite™ 640/660 EV Membrane Staining Kit, 100 labelings

1KIT

Biotium

31000-T

EvaGreen® Dye, 20X in Water

1mL

Biotium

31003-1

FastEvaGreen®MasterMixforqPCR(500rxn)

5x1ML

Biotium

31028-T

AccuClear® Ultra High Sensitivity dsDNA Quantitation

 Kit with DNA Standard, trial size (200 assays)

1kit

Biotium

31041-1

Forget-Me-Not™ EvaGreen® qPCR Master Mix (500 reactions)

5x1ml

Biotium

31069-T

AccuGreen™ Broad Range dsDNA Quantitation Kit (100 assays)

1kit

Biotium

31077-T

EvaGreen®Plus,20XinWater,1mL

1ML

Biotium

33021-1

GloMelt™ Thermal Shift Protein Stability kit

1kit

Biotium

33021-T

GloMelt™ Thermal Shift Protein Stability kit

1kit

Biotium

33022-1

GloMelt™ Thermal Shift Protein Stability kit

1kit

Biotium

33022-T

GloMelt™ Thermal Shift Protein Stability kit

1kit

Biotium

40002-T

dUTP CF® Dye Conjugates

5 nmol

Biotium

40020-1

5-Aminoallyl-dUTP (AA-dUTP), Lyophilized Powder

1 mg

Biotium

40027-T

dCTP CF® Dye Conjugates

5 nmol

Biotium

40060-T

RedDot™1 Far-Red Nuclear Stain, 200X in Water

25uL

Biotium

41010-1mL

6X GelRed Prestain Buffer with Orange Tracking Dye

1mL

Biotium

41029-50G

GelRed®AgaroseLE

50G

Biotium

90066-1

MTSEA Biotin-XX

50mg

Biotium

E90002

PMA-Lite™ LED Photolysis Device

1EA

Biotium




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